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That is unfortunate.
Dr Tims is a find product imo. He also developed Biospira which I also think works well. They both have their limitations. Fortunately, adding the bottle after bumping up your ammonia shouldn't negatively impact either product.
For instance, in a larger tank it will take much longer to process 2ppm ammonia than in a smaller tank. What size is your tank, and what size bottle did you add?
Nitrifying bacteria are very slow reproducers compared to most other bacteria types. If exposed to high temperatures, the bacteria may not arrive in as good a condition as they otherwise would and may take longer to reproduce to significant numbers. Freezing is what you really need to avoid as that can make the product worthless.
Another key thing if you aren't already doing it is to shine a light at the back of the seachem badge to read it. It can look higher than it actually is if not back lit or if lit with blue reef lighting.
That’s good to know that I didn’t nuke the tank [emoji28].
The tank is 125G with a 55G sump that has 30G when operating. I used a full 120G rated bottle and half of another 120G rated bottle.
Nothing really happened until I add Bio Spira. I think Dr Tim died during shipping as it was over 100 degrees here and I had 2 days shipping. Bio Spira was picked up at the LFS.
The cycle kicked off after adding bio spira. I’m now at 0.25 ammonia, 2.0 nitrites, and about 20 Nitrates using an API test kit.