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I’m starting a phytoplankton culture with the long-term goal of supporting live brine shrimp and copepods, using the phyto as their primary food source. This serves two purposes: first, to provide my reef and its inhabitants with fresh, live nutrition; and second, to eventually maintain a stable enough pod population to responsibly keep a dragonet. My aim is to have a fully established live-food ecosystem before introducing the fish.

This thread will act as a running log of the entire process for anyone who wants to follow along.

Supplies Used
• Aquatop Breza AP-30 air pump
• 3/8" air hose
• 3/16" rigid plastic tubing
• Vessels
• Phytoplankton starter culture
• F/2 fertilizer
• 100 ft LED light strip
• Airline control valves

The Vessel
For the first vessel, I repurposed a 2-gallon glass jar that previously served as a terrarium. One advantage of using this jar is that it already had lighting installed, which saves a bit of setup work. However, reusing a jar like this comes with potential risks, and it’s important to consider them before committing to the build.

The existing bulkhead fitting and nozzle will add some convenience to the system, but they also introduce possible failure points. A compromised seal could easily result in two gallons of green “liquid salad” on the floor. After a thorough inspection and leak test, I’m confident the seal on this jar is solid enough for now—but I’ll still be monitoring it closely. In the future, I may switch to fully sealed glass jars, but for the moment, I’m sticking with this setup.

Sterilization
To prep the jar, I scrubbed and rinsed it thoroughly with 99% isopropyl alcohol. Once cleaned, I dried it with a paper towel and allowed it to sit for approximately 24 hours to ensure all alcohol had evaporated before adding any water.
20251106_161454_A12B3824-B43E-44E7-8CB0-4783B17B8FDE.png



20251106_161704_BEFC345C-A1DC-4FD3-9DF8-5311D64F3324.png
 
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Airline Installation Notes

For the airline section, I used 3/16 rigid tubing in combination with 3/8 airline. I started by inserting the rigid tube down into the vessel, making sure to leave several inches extending out through the lid for easy access and connections.
20251118_204509_19ED0708-E780-43C0-B462-7CB361A439FD.png

With the rigid tube positioned, I slipped the 3/8" airline tubing over the exposed end. From there, the airline runs through these components:​
  1. Check valve – installed first to prevent any back flow into the system. 20251118_204508_755DA72B-8794-4795-91BD-7ED18C1CE6CA.png
  2. Airflow adjustment valve – placed after the check valve to fine-tune the air entering the vessel. 20251118_204508_BE93B579-B750-4846-AE0A-CDB23B815580.png
  3. Airway splitter – final connection point, distributing the air supply as needed. (Added later)​

Here is the result! I am eager to get it filled and begin fine tuning.

20251118_204509_9AF4A6D8-E81B-4A7B-A75F-A2C051F8C750.png
20251118_204508_F94CA3CA-9E59-43F8-939C-E799A5B136B4.png
 
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Lighting Setup Log

For the lighting, I used a 100 ft RGB LED strip. To keep the wiring clean and organized, I started by running the power lead straight up the side of the vessel, perpendicular to the direction the strip would be wrapped. This gives the power connection a nice, straight path without crossing over any of the spirals later on.

Once the lead was positioned, I continued guiding the strip all the way up to the top of the vessel. At the rim, I made a neat 90-degree turn and began wrapping the LED strip around the vessel itself. I worked my way around in smooth, even spirals, stopping every few wraps to secure the strip with a bit of tape. This kept everything tight and prevented sagging as I went.

I kept wrapping and securing the strip down the length of the vessel until I reached the spigot area, where I finished off the run. The result is a clean, evenly lit vessel with a tidy power path and a consistent spiral of lighting from top to bottom.
  1. Positioning the Power Lead: I started by running the LED strip’s power lead straight up the side of the vessel—perpendicular to the direction the strip would eventually wrap. This keeps the power connection clean and organized.
    20251118_185145_679652C1-9E72-4469-943C-5281B7F23931.png
  2. Routing to the Top: I continued guiding the strip upward until it reached the top edge of the vessel.​
  3. Making the Turn: At the top, I made a clean 90-degree turn to transition into the wrapping direction. 20251118_185145_2279EEF5-248C-4807-9D17-98982C54149A.png
  4. Wrapping the Vessel: I began wrapping the LED strip around the vessel, working in even spirals. Every few wraps, I added a small piece of tape to keep the strip tight and aligned. 20251118_185146_82BF5828-01B7-4C2F-ADEB-3F4047D2F704.png
  5. Continuing Downward: I repeated this wrap-and-secure pattern all the way down the vessel, stopping once I reached the area around the spigot.
    20251118_185146_2149C3CC-56F4-45E0-A7DE-A92B29699EDE.png
  6. Let there be light: The finished result powered 😀

    20251118_185145_4BA2B8BD-E442-4A44-BE6F-7839744F369F.png 20251118_185146_73C64295-51C7-4F4F-8E22-09CA5D39BD25.png
 
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Reserved for results, additional notes, etc.

Harvest Results:​

DateVolumeAdditional Notes
November 19, 20252 cups (~437ml)Whole harvest was > 1gal < 2gal, rolled the rest into a 2gal culture.
November 28, 20257 cupsWhole harvest was 2 gal, harvested under 1g, rolled the rest into another 2g culture
 
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I’ve also gone ahead and begun the process of hatching brine shrimp, with an additional tank to raise them. I’ll catalogue this as well in time 😀


20251118_205221_9540F94B-4FB1-42A7-8615-00003567DF87.png
 
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Always happy to hear criticism and advice from more experienced folks! :)
 

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I've been thinking about this for the future. Has anyone considered hooking this up with a doser? I'm picturing in my mind something like the dos 2x pump where it adds to the tank and replaces the water used in the culture.
 

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I've been thinking about this for the future. Has anyone considered hooking this up with a doser? I'm picturing in my mind something like the dos 2x pump where it adds to the tank and replaces the water used in the culture.
I did some quick searches and it looks like this has been done before. I'll paste thread links here.

 
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I did some quick searches and it looks like this has been done before. I'll paste thread links here.

Great find! Thank you for sharing! I am definitely considering doing some auto-dosing now, what a great idea.
 
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20251115_083405_723E838C-AE4C-47EE-BDBB-D7FE36D364B6.png
Didn’t forget about this thread! I’ve completed the build, but work has been so hectic I haven’t updated the build thread here
 
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20251119_231720_0BB98688-BF31-47C9-B15B-E9F2EBAF19B9.png
First harvest is in. Two cups to feed my corals and brine shrimp over the next week, as I’ve rolled the rest of the batch into a larger one
 
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Updated my thread with actual content, apologies to anyone who had read this incomplete mess prior. I have some cool things in the works, and some additional upgrades I've done that I'm excited to share.
 
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Tetraselmis Phytoplankton Culture - Day 01​

 
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Tetraselmis Phytoplankton Culture - Day 02​


 
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Artemia Franciscana Culture - Day 01

• Culture started on November 21, 2025.
• Hatchery was made using one of these kits, a two liter bottle, and an air pump
• Brine shrimp eggs were purchased separately from the kit -- The kit includes some egg & salt mix to start a culture; but I don't like the idea of having parameters out of my control. I opted to mix my own water with a SG of 10.024 using Instant Ocean classic
• I am hoping to have a number of my shrimp reach maturity in order to gut load and live feed to some of my fish.
• I will also use baby brine shrimp to feed my corals





20251122_150426_DCBEBCD8-2ABF-45DC-992E-F74481A4517B.png
(Left) Brine Shrimp tank with a couple of air stones and a heater. This setup has since been altered to use an air stone since, The air stone is tuned better for less micro-bubbles.
(Right) The brine shrimp hatchery kit that I had purchased. Digital thermometer in front of that.


20251122_150426_7B16B091-22C8-431D-A64A-3E084AA52AC7.png
A shot of various brine shrimp congregating on the glass towards a light source.
 
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Tetraselmis Phytoplankton Culture - Day 03​


 

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