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Without wanting to really debate this - it seems that you're ignoring the elephant in the room. When fish are shipped to an LFS - there are estimates that 30% (minimum) are DOA - at least at some bad LFS - bad shippers. You are now trying to criticize people that are using fish to cycle an aquarium? You can not use a large blue tang to cycle a 10 gallon tank. You can use a small clown fish to cycle a tank - with no damage - the studies have been published previously on this site - there is no reason not to do so. Except - some people seem to want to take months to do something that takes days. Here is the proof. The TTM method. If you change your water often enough - you can cycle a tank with fish in the tank. There is no biological filtration in the TTM method right? so - do it on a smaller scaleAl right then. Lay out your experiment in detail. What parameters will you look at precisely. What will be the "proof" that you will determine. I don't mean peers like other hobbyist, I mean peer review that you would go through to publish a paper in a real scientific journal, just like you advance. I just don't see how you will come to a conclusion on the way you asked the question. It is like asking if something causes cancer. What other variables come into play? How long will you keep the study going to determine if it does or not? I guess my real question after this long post is why? Just because you can do an experiment, doesn't mean you should. Why would you want to "possibly" hurt an animal if it is not necessary to start up an aquarium. Seems to me you are taking one step forward and two steps back.