I believe you base the amounts on your tank size. Follow the instructions on each additive. Some people add more of certain things then others, just tweak it as you go as there is no one size fits all for this.Would someone kindly post the amounts they add to their culture tub? I have most of the ingredients just want a rough amount to add. I will make 500ml with mb7, reef roids, vinegar or no pox and amino for my 150g. Thanks in advance