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I read the abstract which did reinforce there is significant nutrient uptake thru holdfast. I find it interesting, that nitrate was the molecule absorbed in substrate and not ammonia. Unfortunately, the link was the “abstract only” as I searched for details on how experiment was conducted.What I've read is that while most macroalgaes don't use holdfasts for much, many of the rooted siphonaceous species (caulerpa, halimeda, udotea, penicillus, etc) do use them for a significant part of their nutrient uptake. Here's a study I found about Caulerpa prolifera doing this - https://pubmed.ncbi.nlm.nih.gov/32175590/
Lasse,Add me to this list
My refugium is something like this - but I did not incorporate soil in my mix of the DSB in my refugium. However I have reverse flow through the sand bed of around 300 L/hour and I injected skimmate in the plenum below the sand bed when I started up. I inject around 24 ml 8% ethanol in the plenum every day. For the moment I add a water flow for 30 minutes each hour and if my redox is above 0 - I add ethanol. In this way I alter between aerob and anaerob environment. With other words - between reduction and oxidation.
See below ORP lower than 0 = reduction - higher than 0 = oxidation
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My aquarium is a heavy loaded 300 L system and I can control my NO3 concentrations this way. I control - for the moment - my PO4 through GFO. My GFO reactor have an apartment with filter floss before the GFO in order to keep organic matter (and hence bacteria growth) away from the GFO. I have a skimmer. There is a lot of no photosynthesising but filtrating animals in my tank, sponges, yellow sun coral, small feather dusters (at places there my copper band can´t reach them) - even tunicates grow in some places. There are sediments ending up in some places - and I never clean it up. There is tones of cryptic zones in the display. I do not have Zostera (eelgrass) but I know one place there my friends have succeeded with this tricky plant in a DSB based system.
The aquarium is nearly 10 years old
If you want to use a bacteria driven system - you should maybe add a skimmer and use the skimmate in order to feed your filtrating organisms - there is no better place for bacteria growth than the skimmer cup! In order to fight one type of cyanobacteria mats I use marine snow (fine CaCO3 powder) that have been soaked in skimmate for half an hour. And my cyanobacteria mats slowly disappear.
Good luck with your experiment and keep on thinking outside the box - inside the box will always result in stagnation!
Sincerely Lasse
A young couple who was excited to grab up a bunch of my Dictyota coriacea from my temperate tank last year. They were working on a study of nitrogen transport in marine algae and plants, although now that I think about it, it may have been carbon... Whatever... The point is... wait, I am pointless.I read the abstract which did reinforce there is significant nutrient uptake thru holdfast. I find it interesting, that nitrate was the molecule absorbed in substrate and not ammonia. Unfortunately, the link was the “abstract only” as I searched for details on how experiment was conducted.
A friend with scientific background sent me the rest of the article on Caulerpa Prolifera. Amongst other things, the submerged part of holdfast favored uptake of nitrate to ammonia. Caulerpa Prolifera sequestered both inorganic & organic nitrogen & phosphorus in submerged part of holdfast and that was NEW information to me.A young couple who was excited to grab up a bunch of my Dictyota coriacea from my temperate tank last year. They were working on a study of nitrogen transport in marine algae and plants, although now that I think about it, it may have been carbon... Whatever... The point is... wait, I am pointless.
Your tank excites me, @Paul B's tank excites me, @Lasse tank excites me, This experimental tank excites me. Anyone that does anything beyond the "norm" makes me smile and is interesting to me.
Thinking outside the box is a road of many wrong turns, but it is a road to success.
Still pointless and rambling....
Skip the ghost feeding if you start with mulm from another aquarium - get a fish and feed sparely - like my suggestions hereI should add that I'm cycling it with mulm from another tank + fish food
I appreciate your advice and I know you're very very experienced but I'd rather use the method that I prefer and that I've successfully used on all of my tanks.Skip the ghost feeding if you start with mulm from another aquarium - get a fish and feed sparely - like my suggestions here
Sincerely Lasse