Sorry but I am still confused.
With respect to the original post, which I understood as basically asking what type of hardy fish other than a damsel can be used for cycling a tank. Although I am not going to quote the exact question, I am of the understanding that this was the crux of the question.
Reading through the thread I was relieved that the vast majority of the answers and comments did not just include a list animals that unfortunately are sometimes classified both hardy and expendable, the usual suspects belonging to the Family Pomacentridae, including Clowns and Damsels.
Thankfully the majority of the comments where inclined to discuss the Nitrogen Cycle itself and the many, many methods of developing an effective biological filter to convert toxic ammonia to less harmful compounds / elements. Also the effects and levels of these elements on the animals involved.
I read through many, including as always very informative thread
@Lasse , but unfortunately not everyone's comments, so I apologize for any that I missed.
I am not trying to disprove or relegate any of the other methods that have been discussed. All I am saying is it is prudent to use a simple and effective precaution before adding the actual livestock.
One can study the data and read as many articles as one wishes, but with regards nature one must always be aware of its sometimes unpredictable path, it will often throw a side ball. That is why I believe the simple precaution of loading, stressing and testing without a live sample is prudent especially with regards the animals welfare.
Creating a diverse biological environment is in my opinion another topic altogether and could become rather distracting especially for newcomers to the hobby when discussing basic biological filtration (CYCLING A TANK)
I still can not see why so many people are reluctant to practice the simple procedure of using a measured ammonia source and test to validate the correct operation of a basic biological filter to convert a given ammonia load to less toxic forms of nitrogen, before the addition of livestock. Test ammonia level, add measured ammonia source, test ammonia source depletion time. If there is no evidence of an active biological filtration then add one in the form of Nitrifying Bacteria, (preferably and most commonly some form of sufficient surface area and a bacteria source would have been made available prior to testing the filter) ,once again readily available from the many manufacturers following the findings and patents of Dr. Hovanec. You can then follow any path you wish once this is proven. Even the most elaborate of methods include the addition of an ammonia source, be it fish or decaying organics. Surely using a form of ammonia that can be simply calculated and therefore more accurately compared just makes common sense. This can be tested in as little as 24 hours without any risk to livestock.
For those who have neither the inclination or time to read the remainder of my comments, then I don't blame you :) the above summary concludes my comments. Happy Reefing and Best Wishes from the UK to our fellow hobbyists where ever you abode.
Continued......
The main reason I love to participate with this forum is to keep up with my fellow reefers trials and tribulations, but to be honest my favorite part is to look at the photos of these amazing animals and aquariums. However I also appreciate the excellent articles by both qualified and experienced hobbyists.
My main interest over the past 30 -40 years has always been in respect to Aquatic Biology. As a consequence of my professional duties, as far as aquarium keeping is concerned. I have had large breaks in the hobby . So each time I start up again, I start a fresh as a novice just to keep up with modern methods and not be drawn into the comfort of "It works so why change it". I have seen huge strides since the Berlin and Dr Jaubert methods.
Although I have no academic qualification in this field. I do prefer to access most of my scientific information from my Logon to Researchgate and the many excellent professional journals.
As was pointed out to me by another member. I am very much aware of Peer Review and the importance of reference and citation. unfortunately many articles are not subject to peer review so must be taken in context. Also the vast majority of those that are peer reviewed are unfortunately not directed at the aquarium hobby or the keeping of ornamental species so I believe context is particularly important as is reading the whole article not just the abstract or conclusion.
As a professional with licences to import, aquaculture, and sell Marine Ornamentals in the United Kingdom, it is a stipulation of those licences to first certify an active biological filter before adding livestock and to maintain SOP's to ensure its operation is successfully maintained at all times. If I did not adhere to these stipulations, I would be in breach of at least one of those licences. In my profession it is not just classed as bad practice, to use livestock to routinely cycle an aquarium, it is a legal duty not to do so.
If you read all the way to this point, I am impressed, thank you for your patience.:)