PH Testing question

FernBluffReef

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I have a question about PH testing that I'm hoping someone can shed some light on.

RedSea 112 Gallon display / 31 Gallon sump. 14 months new, started with macro rock. Well stocked with fish. Couple clams, moderate coral mostly LPS and a large nem.

PH was historically averaging ~7.7-7.9 in the AM using a Hanna colorimeter. As a result, I constructed myself a DYI Recirculating scrubber to raise the PH which is now working great

Weekly tank testing results from this morning
- According to Hanna colorimeter HI790 reads 8.5 which I believe to be high as the Hanna colorimeter has always been around .2 higher than any of my other testing methods. The colorimeter never reads less then 8.3 since installing the CO2 scrubber.

- PH controller in-tank - Milwaukee MC122 PH controller - says 7.9

- Hanna PH checker (HI98103) - hand held probe reads 8.3

- So basically, all over the Map. All of the above are rated +/- .2 accuracy hence 7.9 to 8.5 seems like a wide range

I just got the PH checker above from another reefer last weekend for a deal, so I can quickly test all my tanks. I calibrated it last weekend and checked the calibration of the Milwaukee at the same time - both with the same calibration fluids pre-warmed in the tank. This was during the day and both the newly calibrated Hanna PH checker, and the Milwaukee read the exactly same at that time.

The CO2 scrubber is set to turn on right at the threshold of 7.9-8.0 and shutoff right at the threshold of 8.2-8.3. It's on this morning as to be expected, and I tend to believe the in tank reading from the Milwaukee vs. the others.

Question, is there really expected to be that much difference between a stable probe in-sump vs. a real-time reading with a colorimeter or handheld probe? The in-tank slowly goes up during the day as expected and slowly down once the lights go off as expected. The other testing methods seem to read about the same day or night save if tested first in the morning and peak of the afternoon when you expect to see a greater difference.

I know I should not worry too much about PH but given I went thru all the effort to build and install the recirculating CO2 scrubber at least want to know its working - it is:) . I also know, one should generally pick one test method and stick to, my only real reason for continuing to test PH as part of my weekly testing is to help identify when the in-tank probe maybe starting to drift and either needs recalibration or replacement. As of right now it's only ~30 days new probe and again when I calibrated the Hanna checker handheld probe, I also double-checked the Milwaukee with the same fluids, and it did not require any adjustments. Using two point calibration 7.01 and 10.01 calibration fluids


Thanks for the insight

P.S. ~ 2 weeks ago, I tested with a RedSea PH test I have around in the morning. It read about the mid-point between the Milwaukee probe and the Hanna Colorimeter at that time.
P.P.S. I tested my other tank this morning with the Hanna PH checker and it read 8.1, so .2 lower than the main display which is largely consistent with what I'd expect - that the other tanks PH would be quite a bit lower as it does not have a scrubber.

Full test results from this morning if adds any value
- Salinity 35.1
- Temp 76.50
- Nitrate 9.6
- KH 7.7 (a bit low usually I shoot for Low 8s and understand helps with PH stability)
- Phosphate .09
- Calcium 441
- Mag 1,445
 

Randy Holmes-Farley

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It can be quite challenging to know which of multiple tests that disagree is accurate, if any.

If you put all three into the same calibration fluids, do they all read them correctly?
 
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FernBluffReef

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It can be quite challenging to know which of multiple tests that disagree is accurate, if any.

If you put all three into the same calibration fluids, do they all read them correctly?
Yeah, if two of the tests were close that would make it easier to pick a compromise but no. As mentioned, I just calibrated the two probes last Sunday using the same two calibration packets 7.01 and 10.1 that were pre-warmed in the tank. At that time, they both measured the exact same #s which was 8.3 at the time I calibrated. This morning (5:30 AM before lights on which is when I usually test) those same two probes - intank says 7.9 and handheld 8.3 and of course the colorimeter 8.5. The one I 'most' believe is the intank probe at 7.9.

I'll pop the handheld back in some calibration fluid later today and see how close it is to the original calibration I performed last Sunday. The handheld of course is stored but I did store it in some 4.01 calibration fluid per the instruction to keep the bulb damp so I'd hope it didn't vary that much in less than a week of non-use. That and when the lights have been on, and PH higher towards the late afternoon the tests seem to be more consistent but that isn't when I usually test LoL

Thanks Randy for the thoughts
 
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FernBluffReef

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Well testing again, later in the day, didn't help a lot save I continue to distrust the Hanna Colorimeter...and believe that the Milwaukee is correct. Although the majority of tests say my trust of the Milwaukee may be misplaced :-(

Detail

As a reminder, very early this morning - before lights come on - tank 76.5 degrees
- Recently calibrated Milwaukee PH probe in sump. PH = 7.9. Probe is new as of December, two point calibrated upon install with 7.01 / 10.1 and checked again last weekend with no adjustments needed/made
- Hanna PH Checker probe - calibrated last weekend at the same time as the Milwaukee also two points - PH 8.3. I just acquired this checker from another reefer last weekend. The bulb had been left dry, I rehydrated for a couple hours, calibrated and it read consistent with the Milwaukee when calibrating hence I was left happy with it
- Hanna PH Colorimeter - PH 8.5


This afternoon, tank is 77.4 degrees
- Milwaukee probe in tank measures 8.2. It's about 3PM which is about the time it normally gets to peak and shuts off the CO2 scrubber so it's likely really really close to 8.3. Specs say the Milwaukee probe is +/- .2
- Hanna Colorimeter says 8.6, but flashes error to check sample prep indicating the sample is out of range. Specs say the Hanna test is good for PH 6.3 - 8.6 +/- .2 Reagent expiration 11/2027
- RedSea says 8.6 - Spec says the RedSea test is good for PH 7.6 - 8.6 +/-.1 Reagent expiration 10/2025
- Hanna checker handheld PH probe says 8.4 the first time and 8.5 there after - so somewhere between 8.4 - 8.5. Specs say probe is +/- .2 and can read from 0 - 14. Reminder both the Hanna and Milwaukee probes were calibrated in the same solution last weekend
- I went ahead and re-checked the Hanna checker handheld PH probe in some room temp left over 7.01 calibration solution I sealed from last weekend. Measured 7.3. ugh Checked again with a new satchel of warmed to tank temp 7.01 buffer , measured 7.3 at first eventually 7.2. I did not pull the Milwaukee probe as again it was just calibrated upon installation in December and I just checked last weekend when calibrating the Hanna checker probe not needing any adjustment


Freshly mixed seawater from last night - temp 67 degrees
- Hanna Colorimeter says 8.6 but flashes error to check sample prep
- Hanna checker handheld PH probe says 8.6 - 8.7
- RedSea says 8.6
- Hey Fresh mixed seawater is the only consistent test all at 8.6-8.7 ugh


So both the colorimeter tests say the tank is currently 8.6-8.7 which I do not believe full stop
- The Hanna probe is reading roughly .2 high per both the 7.01 calibration solution used last week and sealed and a new satchel

So where am I left..... I'm tending to trust the Milwaukee PH Probe that is in the tank controlling my CO2 scrubber. When adjusting the Hanna probe by -.2 as reference to the 7.01 standard that tells me it should be reading 8.2 - 8.3 which is exactly what the Milwaukee say.

My consternation is the Milwaukee is consistently reading up to .5 lower than the other references and only matches the Hanna probe if I adjust the readings based on new calibration fluid - but again the Hanna checker probe had been dry before I got it so there is that - I'm not ready to buy a new probe for it quite yet though :)

I guess, most importantly corals and fish are happy - me and my testing on the other hand LoL -
 

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