Randy Holmes-Farley
Reef Chemist
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My Tank Thread
As far as denitrification goes in a reef tank. If I could explain this correctly, war would start in the hobby. Denitrification in a reef tank does not happen, or at least not at the rate people assume. All these things people repeat in the hobby is simply that a "repeat". Wanna see how it works? Take water from your tank that has Nitrates of like 100. Setup 3 tanks: Seal completely tank 1. Create opening of 3% for tank 2 (my tank), and leave tank 3 fully uncovered (most tanks in the hobby). Measure Nitrates after 1 month, 6 months and 12 months. You will be surprised how things actually work. In a sense ocean and reef tanks are simpler than is generally assumed.
FWIW, I make no assumptions about how much denitrification takes place in reef tanks. Only thing I am confident of is that it is variable based on levels of organics and amount of accessible places low in O2.
As to that proposed experiment, I'm not sure I understand it (what is in these tanks?) or if it is something you have actually tried, but it seems to have a problem, IMO. Denitrification requires organic matter to degrade in low oxygen zones. To deplete 100 ppm of nitrate would take a very considerable amount of organic matter (if it was methanol, it would take 52 ppm). Since reef tank water typically has on the order of 1 ppm of total organic carbon, your experiment as I understand it is set up to fail due to lack of sufficient organic carbon.