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It's not real clear why acriflavin treats brook, but when it comes to velvet, the only thing it's treating is the secondary bacterial infections that can cause just as much damage as the velvet itself. It's only a single step in the protocol to cure velvet.
+1 IME; there is no single step to treating velvet which works consistently.
I've had the highest success rate using this three step method:
- 5 min FW dip. This clears the gills (so the fish can breathe properly) and forces 80-90% of the parasites to drop off the fish. This critical step is important because it gives the fish a fighting chance going into QT.
- 90 min acriflavine bath. All those bite marks, where the parasites had been feeding, leave behind tiny holes just waiting to get infected. An antiseptic, such as acriflavine, helps to prevent this from happening.
- 30 days in either copper or CP. This finishes off whatever parasites remain. Neither provides any immediate relief to the fish; but after the remaining trophonts drop off, encyst and try to reinfect your fish as free swimmers... copper or CP is in the water to zap them.