I wrote this in another thread dealing with ammonia badge and prime but figured I would add here as well. Sadly I lost my Copperband on day 15 of QT. This is a complete description of what I did in QT.
"Unfortunately lost the copperband sometime during the night. I had dosed Prime again last night because badge was light blue/light green again depending on light. I have read and was attempting to follow multiple quarantine articles. This is my second time setting up QT. Last year when I was setting up my nano tank I used live sand and rock though and was told that was a no no. We used Prime a couple times the first week of this QT a year ago but then didn't have to. Only lost 1 fish out of 6 and that was due to an accident and not disease. Never had to dose any meds. Right now I am in the process of setting up 180g tank and wanted to get a jump on QT so I could do a longer QT, and have a couple fish comfortable and ready to go once the big tank was ready. I had an airstone driven sponge filter in my sump of my nano tank for one month and used Microlift special blend as a boost to my DT sump. I set up the QT tank with this sponge filter, HOB filter, jabeo nano powerhead and HOB skimmer (not necessary but was just sitting there from before we had a sump on DT) I also added microbe lift and To Little Fishes BioPronto. QT was set up for 3 days and then added a Copperband and Bartletts Anthias along with Two Little Fishes Bactiv8 and Hydroplex. Bartlett Anthias did not look well when released from bag (Fish store is two hours away). Drip acclimated for 1 hour and then added to QT. Bartlett died the next morning. I never saw anything obvious wrong with it other than breathing heavy and hiding/laying on bottom next to tube. Ammonia was 0.25 or less with ATI at this time, 0 nitrite, <5 Nitrate. Copperband ate and was very active for 10 days. Performed 4 waterchanges during this time but ammonia was 0.5 per ATI so added 2 doses of prime 48 hrs apart along with all the bacteria products. Nitrite still 0 and Nitrate 5. Added carbon to QT day 4. Added 3 lyretail anthias from Divers Den on Tuesday the 19th after dipping in hydroplex for 10min and ammonia badge. (Day 10 for Copperband) Ammonia badge was light green the morning after adding so added another dose of Prime. That evening Ammonia badge was reading in the light blue range so completed a waterchange with treating 12 gal new water. (QT is 20g not sure if I mentioned that) ATI was reading very Green. Repeated this for next 3 days. Badge always reading lt blue or lt green. Copperband was listless and did not eat yesterday and began "piping" at the surface in the evening. Copperband also had a few brown almost bruise looking spots and two white spots on fin. I pulled carbon and dosed 1 dose of metroplex and Kanaplex. Copperband looked to have more spots on fins so changed directions and completed a 12g water change then added 1ml of Cupramine. It was after 11pm and as my kids home Christmas was in the AM I went to bed after observing for about 30minutes. Copperband was dead when got up at 6am. The 3 lyretails are swimming fine and eating. I do not see any spots or marks on them. I added a dose of prime because ammonia badge light blue. Will complete a waterchange once water is at temperature. Now I don't know if I should add Prime. ATI is very green. Should I continue to add Cupramine in case the copperband had something? How should I proceed? I am at a loss... And please if I did something wrong in all this please let me know. I don't want to keep killing fish. This is the first time I attempted to dose any medications in saltwater. I have in Fresh though."