If You're Serious About Lighting, Know This

Dana Riddle

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Per @GoVols suggestion:

Lighting for reef aquaria can be as simple or complex as you wish to make it. If you’re really serious about lighting, these are some of the terms you might want to be familiar with:

Accessory Pigment: A photopigment that absorbs light and transfers it to chlorophyll a and hence reaction centers. Accessory pigments in zooxanthellae include chlorophyll c2, peridinin, beta-carotene and a few others.

Chlorophyll: An organic compound with a magnesium cluster that is capable of absorbing light. There are a few chlorophylls. Zooxanthellae in corals contain chlorophylls a and c2 (not chlorophyll b!)

Compensation Point: When light intensity is sufficient to produce enough oxygen to meet the respiratory needs of the organism. Compensation points of zooxanthellae are usually less than 50 microMol/m2/sec.

DLI (Daily Light Integral): The total number of photons that promote photosynthesis falling upon an area of one square meter per second. If we think of PPFD as the number of raindrops falling upon 1 square meter per second, DLI would be the inches of rainfall per photoperiod.

ETR (Electron Transport Rate):
The rate of photosynthesis and is the flow of energy between Photosystem II and Photosystem I. Interruption of this flow can create destructive pressures that can destroy a zooxanthella cell. ETRs are measured using a PAM fluorometer.

Nanometer: One billionth of a meter.

PAM Fluorometer: Pulsed Amplitude Modulation Fluorometer or ‘photosynthesis meter.’

PAR (Photosynthetically Active Radiation): The bandwidth of radiation that promotes photosynthesis. It is generally those wavelengths falling between 400 and 700 nanometers, although some ultraviolet-a wavelengths will make photosynthesis happen. It is reported in units of microMol photons per square meter per second (µmol·m2·sec.)

PUR (Photosynthetically Usable Radiation): That portion of PAR that is highly usable in photosynthesis. PUR varies among organisms. Zooxanthellae can utilize some portions of green radiation (up to ~550nm) while green plnts can use it to a much lesser degree.

Photoinhibition: The act of regulating photosynthesis under conditions of high light. There are two types of photoinhibition: Dynamic and Chronic. Dynamic Photoinhibition is reversible response to high light (involving the xanthophylls diadinoxanthin and diatoxanthin. Energy that could be used in photosynthesis converts diadinoxanthin (DD) to diatoxanthin (DT.) In darkness, DT reverts to DD. In case the DD/DT Cycle fails to provide adequate protection, photoinhibition can become chronic. This occurs when excess light energy damages the mechanisms of photosynthesis and energy that could be directed towards growth or reproduction is channeled to repair. Long-term chronic photoinhibition can be fatal.

Photon: A discreet particle of light.

Photoperiod: For our purposes, the number of hours the aquarium receives light, either natural and/or artificial.

Photosynthesis: A complex reaction where light energy converts a carbon source (carbon dioxide or carbonates) into oxygen and simple sugar.

PPFD (Photosynthetic Photon Flux Density): The number of photons that promote photosynthesis falling upon a surface area of one square meter per second.

Reaction Centers:
Where photosynthetic reactions occur.

Saturation: Saturation occurs when increasing light intensity no longer increases the rate of photosynthesis. Zooxanthellae saturation generally occurs at a PAR value of 300 to 400.

Wavelength: A measure of a wave from crest-to-crest (or trough-to-trough.) Light wavelengths are measured in nanometers.

Xanthophylls: Literally ‘yellow leaf.’ Organic compounds that, for our purposes, protect zooxanthellae/coral host from excessive light by shunting light energy away from the photosynthesis process. In corals, these include diadinoxanthin and diatoxanthin. These absorb blue light.
 
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GoVols

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Per @GoVols suggestion:

Lighting for reef aquaria can be as simple or complex as you wish to make it. If you’re really serious about lighting, these are some of the terms you might want to be familiar with:

Accessory Pigment: A photopigment that absorbs light and transfers it to chlorophyll a and hence reaction centers. Accessory pigments in zooxanthellae include chlorophyll c2, peridinin, beta-carotene and a few others.

Chlorophyll: An organic compound with a magnesium cluster that is capable of absorbing light. There are a few chlorophylls. Zooxanthellae in corals contain chlorophylls a and c2 (not chlorophyll b!)

DLI (Daily Light Integral): The total number of photons that promote photosynthesis falling upon an area of one square meter per second. If we think of PPFD as the number of raindrops falling upon 1 square meter per second, DLI would be the inches of rainfall per photoperiod.

ETR (Electron Transport Rate): The rate of photosynthesis and is the flow of energy between Photosystem II and Photosystem I. Interruption of this flow can create destructive pressures that can destroy a zooxanthella cell. ETRs are measured using a PAM fluorometer.

Nanometer: One billionth of a meter.

PAM Fluorometer: Pulsed Amplitude Modulation Fluorometer or ‘photosynthesis meter.’

PAR (Photosynthetically Active Radiation): The bandwidth of radiation that promotes photosynthesis. It is generally those wavelengths falling between 400 and 700 nanometers, although some ultraviolet-a wavelengths will make photosynthesis happen. It is reported in units of microMol photons per square meter per second (µmol·m2·sec.)

Photoinhibition: The act of regulating photosynthesis under conditions of high light. There are two types of photoinhibition: Dynamic and Chronic. Dynamic Photoinhibition is reversible response to high light (involving the xanthophylls diadinoxanthin and diatoxanthin. Energy that could be used in photosynthesis converts diadinoxanthin (DD) to diatoxanthin (DT.) In darkness, DT reverts to DD. In case the DD/DT Cycle fails to provide adequate protection, photoinhibition can become chronic. This occurs when excess light energy damages the mechanisms of photosynthesis and energy that could be directed towards growth or reproduction is channeled to repair. Long-term chronic photoinhibition can be fatal.

Photon: A discreet particle of light.

Photoperiod: For our purposes, the number of hours the aquarium receives light, either natural and/or artificial.

Photosynthesis: A complex reaction where light energy converts a carbon source (carbon dioxide or carbonates) into oxygen and simple sugar.

PPFD (Photosynthetic Photon Flux Density): The number of photons that promote photosynthesis falling upon a surface area of one square meter per second.

Reaction Centers: Where photosynthetic reactions occur.


Wavelength: A measure of a wave from crest-to-crest (or trough-to-trough.) Light wavelengths are measured in nanometers.

Xanthophylls: Literally ‘yellow leaf.’ Organic compounds that, for our purposes, protect zooxanthellae/coral host from excessive light by shunting light energy away from the photosynthesis process. In corals, these include diadinoxanthin and diatoxanthin. These absorb blue light.
Thank You. :)
 

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This is great info!
 

JonasRoman

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@Dana Riddle , thanks for a post with valuable and high density of knowledge.
Can i ask you a question?:

You do not mention PUR? Is it correct that PUR is that part of the PAR which falls within absorption-spectra of chlorophyll a, c,, or at least that part of the PAR which leads to Photosynthesis? In that case, PAR is very exaggerated by some, as if we for instance have a lot of green and yellow light (with low photosynthesis activity, as with some white diods for instance) it must be compensated with more PAR to get equal/proper amount of PUR. Or? Why does we always talking about PAR, shouldn't we always talk about PUR?, thus the interesting part of the light, the PAR with photosynthesis activity so to speak. Why do we not see the different brands showing these figures? I realize that is is more difficult to measure as it should require som selective measure of different wave-length-interval, or?...I realize it is more easy to measure the whole spectrum, 400-700, but says not so much...If we have to measure PUR, how do we do that? With a prisma which is divided the wavelengths and then a light measure of each wavelength? thus not a photometer, but a photospectrometre....Are my thoughts correct??:)

Thanks in advance for answer

regards
Jonas Roman
 
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Dana Riddle

Dana Riddle

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@Dana Riddle , thanks for a post with valuable and high density of knowledge.
Can i ask you a question?:

You do not mention PUR? Is it correct that PUR is that part of the PAR which falls within absorption-spectra of chlorophyll a, c,, or at least that part of the PAR which leads to Photosynthesis? In that case, PAR is very exaggerated by some, as if we for instance have a lot of green and yellow light (with low photosynthesis activity, as with some white diods for instance) it must be compensated with more PAR to get equal/proper amount of PUR. Or? Why does we always talking about PAR, shouldn't we always talk about PUR?, thus the interesting part of the light, the PAR with photosynthesis activity so to speak. Why do we not see the different brands showing these figures? I realize that is is more difficult to measure as it should require som selective measure of different wave-length-interval, or?...I realize it is more easy to measure the whole spectrum, 400-700, but says not so much...If we have to measure PUR, how do we do that? With a prisma which is divided the wavelengths and then a light measure of each wavelength? thus not a photometer, but a photospectrometre....Are my thoughts correct??:)

Thanks in advance for answer

regards
Jonas Roman
Yes, you are correct. I should add several definitions to that list (and will as soon as I can figure out how to modify that post.) I have two Ocean Optics spectrometers and a LS-Cal light source that can be used to make measurements/ calculate PUR. Need to get those items unpacked and get the new lab completed.
 

JonasRoman

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Yes, you are correct. I should add several definitions to that list (and will as soon as I can figure out how to modify that post.) I have two Ocean Optics spectrometers and a LS-Cal light source that can be used to make measurements/ calculate PUR. Need to get those items unpacked and get the new lab completed.
Thanks:). I will follow your thread with great enthusiasm and interest:)
 

Abhishek

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Amazing info .. thanks for posting .

@Dana Riddle - Would like to know what's the ideal photoperiod for an acropora reef tank if PAR is kept within 250-350 and enough water flow provided with stable alkalinity?
Should the photo period vary with the different types of lights used if PAR remains the same? My specific reef is all acros with PAR adjusted to your suggestion using hobby grade Apogee meter but lit only by radium 250 watt halides x 2 .
Its currently running at 5 hours a day but wanted to know whats the ideal PAR photoeriod should be .

Regards,
Abhishek
 
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Dana Riddle

Dana Riddle

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Hi Dana could you give me a recommendation for a spectrometer This is one thing that I have not tried or even looked at
PASCO Scientific has some low cost (but not inexpensive) spectrometers. These are about 1/3 the price of the Ocean Optics specs I have, but I've heard good things about them. Let me know if I can be of further assistance!
 
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Dana Riddle

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Amazing info .. thanks for posting .

@Dana Riddle - Would like to know what's the ideal photoperiod for an acropora reef tank if PAR is kept within 250-350 and enough water flow provided with stable alkalinity?
Should the photo period vary with the different types of lights used if PAR remains the same? My specific reef is all acros with PAR adjusted to your suggestion using hobby grade Apogee meter but lit only by radium 250 watt halides x 2 .
Its currently running at 5 hours a day but wanted to know whats the ideal PAR photoeriod should be .

Regards,
Abhishek
I used PAR of 300 and 10 hour photoperiod to calculate a DLI, and arrived at 10.8, which some be fine. Make changes to photoperiod slowly and watch your corals for any negative signs. The Radiums should generate good coloration and, yes, their PUR will be different from other light sources. Just how much of a difference this makes needs to be investigated, something I could do once the new lab is completed.
 

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