Hmm okay. I have both Dr Tim's and Bio Spira. I thought using it would be worthless since I thought it wouldn't have any time to work since I have to move the fish out on the 4th day. I actually have a sponge in my sump from when I only knew about quarantining the usual way. Then I found out about TTM. I have a Biocube 32 so I really only have room for 1 sponge.There are a couple options:
1. Add bottled bacteria. Yes, it costs money, and you're going to go through a bunch of it doing the TTM, but that's a cost of getting the fish through QT faster than traditional QT. The bacteria you add start to help immediately, reducing ammonia levels.
2. Keep a bunch of bio-media in your DT and transfer smaller bags of it to each clean tank with the fish. They would need to be thoroughly sanitized and re-seeded with bacteria in the DT after each step of the TTM.
3. Monitor ammonia levels religiously, keep freshly made saltwater on hand, and do water changes to reduce ammonia as soon as see levels start to rise. This means using a more accurate tester than API or ammonia badges, because levels can rise quickly in a small tank.