Trident priming

[Joe Rice]

I recently ran the calibration cycle on my Trident and it got me wondering why it was necessary to prime the sample line before and after switching to/from the calibration fluid. Seems to me that the Trident must evacuate the sample line every time it does a test since it doesn't want to test the fluid left over in the sample line from the last test.

So why is priming necessary? @JimWelsh

 

[TheHarold]

I am *guessing* its to maximize accuracy.

After all, the new water does “mix” with the old water in the tube at some level. Extra priming (beyond just the tube length) would purge that small amount of mixing. The vial they use is only so large

 

[JimWelsh]

I defer to Neptune Systems on this one. I'm responding just to acknowledge the mention.

 

[mmorrison55]

Rehashing this topic…

I keep getting test b and etc c failures. Been through the troubleshooting process and had a question related to priming…

When you prime the reagents, ABC, should it include a sample “rinse” as part of the process between filling the curette with reagent?

I just did each individually, and noticed on the reagent primes, that it’s only filling and draining each reagent. There is no sample rinse involved. When I prime the sample, it fills the cuvette 2 times.

Is this normal?it would seem that a rinse between filling the cuvette with reagent would be part of the process.

Im about to reset the trident to factory and try again as I can’t think of anything else to try at this point.

FYI… I just had the trident replaced a few months ago back as part of the replacement option, so it shouldn’t have crapped out this soon, but it seems like it’s drawing sample, and each reagent as it should, so I can’t understand why test b and c would be failing constantly.

 

[mmorrison55]

Update… seems after I opened new bottles of reagent b and c, my new test has been successful.

 

[earleen_nunez23]

Priming and evacuation could be different things—evacuation removes the old fluid, but priming might be conditioning the line for the new stuff. That would explain why both are needed.

 

[Anibal88222]

Evacuating isn't the same as priming though. Getting the old fluid out is one thing, but priming's about saturating the line with fresh sample to make sure residual buildup isn't affecting your readings.