I think it's obvious Neptune has thought about the fact there would be old water in the tube and figured out a solution for it. Nobody wants to be testing three day old tank water. I think it's pretty safe to assume the pump will draw a sample, then run in reverse (for a user programmed amount of time, depending on the length of the hose) to return the water in the line to the sump. If Neptune wanted to get fancy they could time how long it takes to get the sample and then run the pump in reverse for that amount of time +2 seconds or something along those lines.
