Brew12
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My Tank Thread
Let's be clear on this. Freezing doesn't kill all bacteria, but it does kill more than half on the first freeze. Here is a study on repetitive freezing including the bacteria type LRS uses.@Brew12 - Google "Does freezing kill bacteria". You might find it interesting. In fact freezing in general doesn't kill pathogens, it doesn't kill yoghurt or kefir cultures.
http://jb.asm.org/content/70/6/711.full.pdf
And I went to an old conversation with Larry and pulled this out.